Frozen Section Procedure

One of the commonly used fixation methods for frozen tissue sections is to immerse the slides in pre cooled acetone 20 c for 10 min.

Frozen section procedure. The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. Usually used with oncologic surgery rapid diagnosis can guide intra operative patient management. The pathologist evaluates and examines the specimen.

Pour off the fixative and allow acetone to evaporate from the tissue sections for 20 min at room temperature. A pathologist is necessary for the intraoperative consultation. The technical name for this procedure is cryosection.

This procedure is most commonly used in oncological surgery. 1 ride the block as the block descends toward the brush the brush keeps pace with the block by gently resting on the bottom 2 3 mm of the block. 2 gently lift up while crossing the blade as the brush and block meet the blade the section will begin to form under the brush.

This biopsy is then given to a pathologist a doctor who examines tissues and uses laboratory tests to make a diagnosis. The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. Immunostain frozen tissue sections steps 9 28.

Hematoxylin is a natural dye derived from the haematoxylon campechianum logwood tree a tree native to campeche s mexican state. H e is the most commonly used of all the various staining methods available in frozen section. There it is placed on a freezing microtome machine where the bottom of the sample is frozen within seconds.

A razor thin slice of tissue is extracted from the frozen section prepared on a slide and placed under the microscope for review. H e is simple to perform inexpensive and reliable. The cryosection procedure is also known as the frozen section procedure.